Anti-Histone H3 (acetyl K122) antibody (ab33309)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K122)
- Suitable for: WB, ICC/IF
- Reacts with: Human, Arabidopsis thaliana
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (acetyl K122) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K122) -
Host species
Rabbit -
Specificity
This ab shows no cross reactivity with acetyl K56 in Western Blot. Slight cross reactivity with acetyl K56 may be observed in Dot Blot. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Arabidopsis thaliana
Predicted to work with: Mouse, Drosophila melanogaster, a wide range of other species, Brassica oleracea
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Immunogen
Synthetic peptide corresponding to Human Histone H3 aa 100 to the C-terminus (acetyl K122) conjugated to keyhole limpet haemocyanin.
(Peptide available asab34466)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (acetyl K122) antibody (ab33309)All lanes : Anti-Histone H3 (acetyl K122) antibody (ab33309) at 1 µg/ml
Lane 1 : HeLa Histone Preparation Nuclear Lysate - Butyrated
Lane 2 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K122) peptide (ab34466) at 1 µg/ml
Lane 3 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Histone H3 peptide - unmodified (ab34467) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 11.2 kDa (possible cross reactivity)ab33309 shows very slight cross reactivity with Histone H4 in western blot.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K122) antibody (ab33309)ICC/IF image of ab33309 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33309, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - Anti-Histone H3 (acetyl K122) antibody (ab33309)All lanes : Anti-Histone H3 (acetyl K122) antibody (ab33309) at 1 µg/ml
Lane 1 : HeLa Histone Preparation Nuclear Lysate - Butyrated
Lane 2 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K122) peptide (ab34466) at 1 µg/ml
Lane 3 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Histone H3 peptide - unmodified (ab34467) at 1 µg/ml
Lane 4 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K56) peptide at 1 µg/ml
Lane 5 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Histone H3 peptide - unmodified K56 (ab73002) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33309 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K122) antibody (ab33309) This image is courtesy of an anonymous Abreview.Immunocytochemical analysis of Arabidopsis thaliana root cells, labeling Histone H3 (acetyl K122) with ab33309. Cells were paraformaldehyde fixed and blocked in 3% BSA for 1 hour at 4°C. Incubation with ab33309 (diluted 1/1000) was for 18 hours at 4°C.
