Anti-GRP94 antibody (ab52031)
Key features and details
- Goat polyclonal to GRP94
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRP94 antibody
See all GRP94 primary antibodies -
Description
Goat polyclonal to GRP94 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide:
C-KEGVKFDESEKTKE
, corresponding to internal sequence amino acids 593-606 of Human GRP94 -
Positive control
- WB: NIH-3T3, HEK 293, A549, HeLa and U-2OS cell lysates; IHC: Human liver and uterus tissue. ICC/IF: A431 and HeLa cells. Flow Cyt: A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRP94 antibody (ab52031)Lanes 1-3 : Anti-GRP94 antibody (ab52031) at 0.1 µg/ml
Lane 4 : Anti-GRP94 antibody (ab52031) at 0.3 µg/ml
Lane 1 : U2OS cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A594 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 92 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab52031)Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Flow Cytometry - Anti-GRP94 antibody (ab52031)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab52031)Immunohistochemical analysis of paraffin-embedded human uterus tissue staining GRP94 with ab52031 at 5µg/ml, followed by AP staining. Steamed antigen retrieval with citrate buffer pH 6.
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Western blot - Anti-GRP94 antibody (ab52031)Anti-GRP94 antibody (ab52031) at 0.1 µg/ml + U-2OS cell lysate
Predicted band size: 92 kDa
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Western blot - Anti-GRP94 antibody (ab52031)Anti-GRP94 antibody (ab52031) at 0.1 µg/ml + NIH-3T3 cell lysate
Developed using the ECL technique.
Predicted band size: 92 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab52031)ab52031 staining GRP94 in human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue section underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.
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Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab52031)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
