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Anti-Frataxin antibody [EPR6107] (ab124680)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Frataxin antibody [EPR6107] (ab124680)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6107] to Frataxin
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Frataxin antibody [EPR6107]
    See all Frataxin primary antibodies
  • Description

    Rabbit monoclonal [EPR6107] to Frataxin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Frataxin aa 150 to the C-terminus.

  • Positive control

    • Human heart, Human fetal skin and PBL lysates.
  • General notes

    Mouse and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.19 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6107
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Frataxin antibody [EPR6107] (ab124680)
    Western blot - Anti-Frataxin antibody [EPR6107] (ab124680)
    All lanes : Anti-Frataxin antibody [EPR6107] (ab124680) at 1/5000 dilution (purified)

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human skin lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 19, 23 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-Frataxin antibody [EPR6107] (ab124680)
    Western blot - Anti-Frataxin antibody [EPR6107] (ab124680)
    All lanes : Anti-Frataxin antibody [EPR6107] (ab124680) at 1/1000 dilution (unpurified)

    Lane 1 : Human heart lysate
    Lane 2 : Human fetal skin lysate
    Lane 3 : PBL lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 19, 23 kDa
    Observed band size: 17,30 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Frataxin antibody [EPR6107] (ab124680)
    OI-RD Scanning - Anti-Frataxin antibody [EPR6107] (ab124680)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Frataxin antibody [EPR6107] (ab124680)
    Anti-Frataxin antibody [EPR6107] (ab124680)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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