HRP Anti-Frataxin antibody [17A11] (ab197964)
![HRP Anti-Frataxin antibody [17A11] (ab197964)](https://www.abcam.com/ps/products/197/ab197964/Images/ab197964-242628-anti-frataxin-antibody-17a11-hrp-western-blot.jpg "HRP Anti-Frataxin antibody [17A11] (ab197964)")
Key features and details
- HRP Mouse monoclonal [17A11] to Frataxin
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-Frataxin antibody [17A11]
See all Frataxin primary antibodies -
Description
HRP Mouse monoclonal [17A11] to Frataxin -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Frataxin.
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Positive control
- WB: Human heart mitochondrial lysate and HepG2 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
17A11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-Frataxin antibody [17A11] (ab197964)All lanes : HRP Anti-Frataxin antibody [17A11] (ab197964) at 1/5000 dilution
Lane 1 : Human heart tissue lysate - mitochondrial extract (ab110337)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197964 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
