Anti-Frataxin antibody (ab175402)
Key features and details
- Rabbit polyclonal to Frataxin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Frataxin antibody
See all Frataxin primary antibodies -
Description
Rabbit polyclonal to Frataxin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Frataxin.
Database link: Q16595 -
Positive control
- WB: Jurkat, K562, Raji, DU 145 and HL-60 cell lysates; Mouse heart and liver tissue lysates. IHC-P: Mouse and rat heart tissues; ICC/IF: L929 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Frataxin antibody (ab175402)All lanes : Anti-Frataxin antibody (ab175402) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 4 : DU 145 (human prostate carcinoma cell line) cell lysate
Lane 5 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 6 : Mouse heart lysate
Lane 7 : Mouse liver lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Predicted band size: 23 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frataxin antibody (ab175402)Paraffin-embedded mouse heart tissue labelling Frataxin using ab175402 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Frataxin antibody (ab175402)Immunofluorescence staining of L929 cells stained for Frataxin with ab175402 at 1/100 dilution. DAPI was used as a nuclear counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frataxin antibody (ab175402)Paraffin-embedded rat heart tissue labelling Frataxin using ab175402 at 1/100 dilution in immunohistochemical analysis.
