Anti-Frataxin antibody [18A5DB1] (ab110328) at 5 µg/ml + Recombinant Human Frataxin at 0.0005 µg

Predicted band size: 23 kDa

Immunofluorescent analysis of paraformaldehyde-fixed, 0.2% NP-40 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Frataxin with ab110328 at 1/500 dilution, followed by Goat anti-MouseAlexaFluor^® 594 conjugated  secondary antibody at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).

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IHC image of Frataxin staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110328, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow cytometric analysis using ab110328 at 1µg/ml staining Frataxin in HL60 cells (blue). Isotype control antibody (red).

Immunocytochemistry analysis using ab110328 at 0.5µg/ml staining Frataxin in normal Human fibroblasts (MRC5) (fixed and permeabilized) followed by an AlexaFluor® 594-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml). .