Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10367] to GIT1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GIT1 antibody [EPR10367] - BSA and Azide free
    See all GIT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10367] to GIT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y, HeLa, 293T, brain nuclear extract and fetal brain lysates. Flow Cyt: 293T cells.

  • General notes

    Ab249242 is the carrier-free version of ab156001. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249242 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)
    Western blot - Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)
    All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : G1T1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Human brain tissue nuclear fraction lysate
    Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 84 kDa
    Observed band size: 98 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab156001, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab156001 observed at 98 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab156001 Anti-GIT1 antibody [EPR10367] was shown to specifically react with GIT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265230 (knockout cell lysate ab257965) was used. Wild-type and GIT1 knockout samples were subjected to SDS-PAGE. ab156001 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)
    Flow Cytometry - Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)
    This data was developed using ab156001, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized 293T cells labeling GIT1 with ab156001 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
  • Western blot - Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)
    Western blot - Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)
    All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 3 : Fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 84 kDa



    This data was developed using ab156001, the same antibody clone in a different buffer formulation.

  • Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)
    Anti-GIT1 antibody [EPR10367] - BSA and Azide free (ab249242)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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