Anti-Bid Cleavage Site antibody (ab10640)
Key features and details
- Rabbit polyclonal to Bid Cleavage Site
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Bid Cleavage Site antibody
See all Bid Cleavage Site primary antibodies -
Description
Rabbit polyclonal to Bid Cleavage Site -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide (Mouse)corresponding to N-terminus of cleavage site (59/60).
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Positive control
- TNF alpha treated L929 cells.
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General notes
BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by epitope-specific affinity chromatography. -
Primary antibody notes
BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bid Cleavage Site antibody (ab10640)Mouse L929 cells were treated with 5
µ g/mL actinomycin plus 2.5 ng/mL, 5 ng/mL and 10 ng/mL mouse TNF alpha for 5 hours (Lane 3, 4 and 5). Untreated L929 cells (Lane 1) and cells treated with actinomycin alone (Lane 2) were used as controls. Proteins were resolved from the cells by SDS-PAGE. The proteins were transferred to PVDF membranes. The membranes were incubated with the anti-mouse full length BID antibody (left panel) and ab10640 (right panel) at 1µ g/mL. The signal was detected using a goat F(ab’)2 anti-rabbit IgG alkaline phosphatase antibody at a 1/5000 dilution and the membrane was incubated with CDP-substrate followed by chemiluminescence. The data show that ab10640 only recognizes the cleaved mouse BID and not full length (Right panel). The full length BID was revealed using an anti-mouse full length BID antibody (Left panel). Mouse L929 cells were treated with 5 µg/mL actinomycin plus 2.5 ng/mL, 5 ng/mL and 10
