Anti-MC-2 antibody (ab87470)
Key features and details
- Rabbit polyclonal to MC-2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MC-2 antibody
See all MC-2 primary antibodies -
Description
Rabbit polyclonal to MC-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MC-2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125889) -
Positive control
- This antibody gave a positive signal in Human Skeletal Muscle tissue lysate.
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General notes
Previously labelled as MC5 Receptor.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseHamsterChimpanzeeMacaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).Target
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Function
Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins. -
Tissue specificity
Expressed in the brain but not in the melanoma cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 468599 Chimpanzee
- Entrez Gene: 101143231 Gorilla
- Entrez Gene: 4161 Human
- Entrez Gene: 17203 Mouse
- Omim: 600042 Human
- SwissProt: Q9TT23 Chimpanzee
- SwissProt: P33032 Human
- SwissProt: P41149 Mouse
see all -
Alternative names
- MC-2 antibody
- MC2 antibody
- MC5 R antibody
see all
Images
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Western blot - Anti-MC-2 antibody (ab87470)Lane 1 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 5% BSA)
Lane 2 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 3% MILK)
All lanes : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Lane 2 : Recombinant rat MCP1 protein (ab9780) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesMC-2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam recommends using milk (3%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (0)
ab87470 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-MC-2 antibody (ab87470)Lane 1 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 5% BSA)
Lane 2 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 3% MILK)
All lanes : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Lane 2 : Recombinant rat MCP1 protein (ab9780) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesMC-2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam recommends using milk (3%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
