Anti-Laminin 1+2 antibody (ab7463)
Key features and details
- Rabbit polyclonal to Laminin 1+2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Laminin 1+2 antibody -
Description
Rabbit polyclonal to Laminin 1+2 -
Host species
Rabbit -
Specificity
This antibody is pan-specific and reacts well with all laminin isoforms tested: laminin-1 (alpha-1, beta-1, and gamma-1) and laminin-2 (alpha-2, beta-1, and gamma-1). -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P RatWB Mouse
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Immunogen
Full length native protein (purified) (Mouse EHS tumour).
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General notes
Laminin antibodies are widely used to label blood vessels and basement membranes.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Laminin antibodies are widely used to label blood vessels and basement membranes. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)
Ab7463 staining Laminin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:50 dilution. Hoechst 33258 (blue) was used as a nuclear counterstain.
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Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)Ab7463 staining Lamin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:5 dilution. An Alexa Fluor® 488- conjugated Goat to rabbit IgG was used as a secondary antibody. DAPI was used as a nuclear stain and an Alexa Fluor® 568 phalloidin (red) was used to stain actin fllaments.
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Western blot - Anti-Laminin 1+2 antibody (ab7463)All lanes : Anti-Laminin 1+2 antibody (ab7463) at 1 µg/ml
Lane 1 : Mouse EHS tumor crude extracts
Lane 2 : Rat Heart Crude Extracts
Observed band size: 200,400 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 1+2 antibody (ab7463)Ab7463 staining Laminin in rat spinal cord and dorsal root by immunohistochemistry using paraformaldehyde/paraffin embedded tissue. Pepsin antigen retrieval was performed on this tissue sample. -
Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)ab7463 staining Laminin 1+2 in human MDA-MB-231 by ICC/IF (Immunocytochemistry/ Immunofluorescence). The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 buffer and blocked in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary.