Recombinant human Cathepsin K protein (Active) (ab157067)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Cathepsin K protein (Active)
See all Cathepsin K proteins and peptides -
Biological activity
≥ 1.5 U/mg protein. Activity is lot specific.
One unit is defined as the amount of enzyme that will hydrolyze 1µmol Z-Phe-Arg-AMC substrate per min. at 37°C.
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Purity
> 95 % SDS-PAGE.
ab157067 was purified as full-length proenzyme, then auto-activated at low pH. -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MWGLKVLLLP VVSFALYPEE ILDTHWELWK KTHRKQYNNK VDEISRRLIW EKNLKYISIH NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLKVPLSHS RSNDTLYIPE WEGRAPDSVD YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLL NLSPQNLVDC VSENDGCGGG YMTNAFQYVQ KNRGIDSEDA YPYVGQEESC MYNPTGKAAK CRGYREIPEG NEKALKRAVA RVGPVSVAID ASLTSFQFYS KGVYYDESCN SDNLNHAVLA VGYGIQKGNK HWIIKNSWGE NWGNKGYILM ARNKNNACGI ANLASFPKM -
Predicted molecular weight
26 kDa -
Amino acids
1 to 329
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 5.50
Constituents: 0.41% Sodium acetate, 0.08% DTT, 0.015% EDTA, 0.29% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Cathepsin K protein (ab157067)
Each lane contains 1 ug of total protein during time-course activation at low pH.
Lane 1: Pre-Activation.
Lane 2: 0 min
Lane 3: 1 hour
Lane 4: 2 hours
Lane 5: 3 hours
Lane 6: 4 hours
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SDS-PAGE - Recombinant human Cathepsin K protein (ab157067)ab157067 on SDS-PAGE.
Lane 1: 1 ug.
Lane 2: 2 ug.
