Astana Biomed Group, an authorized Abcam distributor in Central Asia

Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [18A5DB1] to Frataxin
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

Overview

  • Product name

    Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1]
    See all Frataxin primary antibodies

  • Description

    Alexa Fluor® 488 Mouse monoclonal [18A5DB1] to Frataxin

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Frataxin aa 50-250.

  • Positive control

    • Flow Cyt: HepG2 cells.

  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033)
    Flow Cytometry - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033)

    Overlay histogram showing HepG2 cells stained with ab156033 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab156033, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Mouse IgG1 - Alexa Fluor® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Flow Cytometry - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033)
    Flow Cytometry - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033)
    Flow cytometric analysis of Human lymphocytes labeling Frataxin with ab156033 at 0.2 µg/ml.
    Lymphocytes were isolated from Control Patients (red), Freidrich Ataxia Carrier Patients (blue), and Friedrich Ataxia Patients (yellow).
    IgG1 Alexa®488 conjugated Isotype control antibody (black at 0.2 µg/ml).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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