Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human Ephrin A1 protein (ab185393)

Key features and details

  • Expression system: Mammalian
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 3X PBS. After reconstitution aliquot and store at

    General Info

    • Alternative names

      • B61
      • ECKLG
      • EFL 1
      • EFL1
      • EFNA 1
      • Efna1
      • EFNA1_HUMAN
      • EPH related receptor tyrosine kinase ligand 1
      • EPH-related receptor tyrosine kinase ligand 1
      • Ephrin-A1
      • Ephrin-A1, secreted form
      • EphrinA1
      • EPLG 1
      • EPLG1
      • Immediate early response protein B61
      • LERK 1
      • LERK-1
      • LERK1
      • Ligand of eph related kinase 1
      • OTTHUMP00000033242
      • OTTHUMP00000033271
      • secreted form
      • TNF alpha-induced protein 4
      • TNFAIP 4
      • TNFAIP4
      • Tumor necrosis factor alpha induced protein 4
      • Tumor necrosis factor alpha-induced protein 4
      see all

    • Function

      Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.

    • Tissue specificity

      Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).

    • Sequence similarities

      Belongs to the ephrin family.

    • Post-translational
      modifications

      Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

References (0)

Publishing research using ab185393? Please let us know so that we can cite the reference in this datasheet.

ab185393 has not yet been referenced specifically in any publications.

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