Recombinant human PD-L1 protein (Active) (ab182689)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: StrepII tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, ELISA
Preparation and Storage
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Applications
SDS-PAGE
Functional Studies
ELISA
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Form
Lyophilized -
Additional notes
Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
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Images
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ELISA - Recombinant human PD-L1 protein (Active) (ab182689)
Immobilized ab182689 at 5 μg/mL (100 μL/well) can bind Recombinant human PD1 protein (Active) (Biotin) (ab246155) with a linear range of 0.002-0.25 μg/mL.
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Functional Studies - Recombinant human PD-L1 protein (Active) (ab182689)Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind ab182689 with an affinity constant of 0.891 nM as determined in BLI assay.
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SDS-PAGE - Recombinant human PD-L1 protein (ab182689)Human PD-L1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The protein migrates as 35-42 kDa due to glycosylation.
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Functional Studies - Recombinant human PD-L1 protein (Active) (ab182689)Loaded Recombinant human PD1 protein (Fc Chimera Active) (ab221398) on Protein A Biosensor, can bind ab182689 with an affinity constant of 2.2 μM as determined in BLI assay.
