Anti-EMC4 antibody [EPR15081] (ab184162)
![Anti-EMC4 antibody [EPR15081] (ab184162)](https://www.abcam.com/ps/products/184/ab184162/Images/ab184162-216445-anti-tmem85-antibody-epr15081-western-blot.jpg "Anti-EMC4 antibody [EPR15081] (ab184162)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15081] to EMC4
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-EMC4 antibody [EPR15081]
See all EMC4 primary antibodies -
Description
Rabbit monoclonal [EPR15081] to EMC4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, SH-SY5Y, U87-MG and HepG2 whole cell lysate (ab7900); Human brain tissue.
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General notes
This product was previously labelled as TMEM85
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15081 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EMC4 antibody [EPR15081] (ab184162)All lanes : Anti-EMC4 antibody [EPR15081] (ab184162) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-EMC4 antibody [EPR15081] (ab184162)](https://www.abcam.com/ps/products/184/ab184162/Images/ab184162-216446-anti-tmem85-antibody-epr15081-western-blot.jpg)
Western blot - Anti-EMC4 antibody [EPR15081] (ab184162)All lanes : Anti-EMC4 antibody [EPR15081] (ab184162) at 1/20000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15081] (ab184162)](https://www.abcam.com/ps/products/184/ab184162/Images/ab184162-216447-anti-tmem85-antibody-epr15081-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15081] (ab184162)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling EMC4 with ab184162 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-EMC4 antibody [EPR15081] (ab184162)](https://www.abcam.com/ps/products/184/ab184162/Images/ab184162-4-benefits-of-recombinant-antibodies.png)
Anti-EMC4 antibody [EPR15081] (ab184162)
