Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-EMC4 antibody [EPR15080] (ab184544)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15080] to EMC4
Suitable for: IP, WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-EMC4 antibody [EPR15080]
See all EMC4 primary antibodies
Description

Rabbit monoclonal [EPR15080] to EMC4
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293, SH-SY5Y, U87-MG and HepG2 cell lysates; Human brain and ovarian carcinoma tissues; mouse liver tissue.
General notes
This product was previously labelled as TMEM85

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15080
Isotype

IgG
Research areas

Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)

All lanes : Anti-EMC4 antibody [EPR15080] (ab184544) at 1/10000 dilution

Lane 1 : 293 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 20 kDa
Observed band size: 17,20 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)

Western blot analysis of EMC4 in HepG2 cell lysate immunoprecipitated using ab184544 at 1/50 dilution (Lane 1). Lane 2: PBS.

Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-EMC4 antibody [EPR15080] (ab184544)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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