Anti-eIF4A3 antibody (ab264315)
Key features and details
- Rabbit polyclonal to eIF4A3
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4A3 antibody
See all eIF4A3 primary antibodies -
Description
Rabbit polyclonal to eIF4A3 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human eIF4A3 aa 125-175. The exact sequence is proprietary. NP_055555.1
Database link: P38919 -
Positive control
- IHC-P: Human ovarian carcinoma tissue. Mouse renal carcinoma tissue. WB: HeLa and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4A3 antibody (ab264315)All lanes : Anti-eIF4A3 antibody (ab264315) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH/3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-eIF4A3 antibody (ab264315)eIF4A3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264315 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264315 at 0.4 µg/ml.
Lane 1: ab264315 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody (ab264315)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal carcinoma tissue labeling eIF4A3 with ab264315 at 1/200 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody (ab264315)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling eIF4A3 with ab264315 at 1/200 dilution. Detection: DAB. Different lots used in each panel.
