Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 02, 2021

Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14301(B)] to eIF4A3
Suitable for: WB, Flow Cyt, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-eIF4A3 antibody [EPR14301(B)]
See all eIF4A3 primary antibodies
Description

Rabbit monoclonal [EPR14301(B)] to eIF4A3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF7, HeLa, Raji and HepG2 whole cell lysate (ab7900); MCF7 and HeLa cells; Human prostate tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14301(B)
Isotype

IgG
Research areas

Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/1000 dilution

Lane 1 : MCF7 cell line with NFDM
Lane 2 : Hela cell line with NFDM
Lane 3 : Raji cell line with NFDM
Lane 4 : HepG2 cell line with NFDM

Lysates/proteins at 20 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : Goat Anti-rabbit HRP at 1/1000 dilution

Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling eIF4A3 with ab180573 at 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Immunofluorescent analysis of 4% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200.
Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Immunofluorescent analysis of 4% paraformaldehyde Hela cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody at a dilution of 1/200.
Flow Cytometry - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Flow cytometric analysis of 2% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/160 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.
Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Immunoprecipitation of Hela labeling eIF4A3 using ab180573 at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.

All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/30 dilution

Lane 1 : Hela cell lysate with NFDM
Lane 2 : Negative control with NFDM

Blocking peptides at 5 % per lane.
Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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