Antibodies, Proteins, Kits and Reagents for Life Science

Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14301(B)] to eIF4A3 - BSA and Azide free
Suitable for: IHC-P, IP, ICC/IF, Flow Cyt, WB
Reacts with: Rat, Human

Product name

Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free
See all eIF4A3 primary antibodies
Description

Rabbit monoclonal [EPR14301(B)] to eIF4A3 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human prostate tissue.
General notes
Ab236131 is the carrier-free version of ab180573. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236131 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14301(B)
Isotype

IgG
Research areas

Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)

Immunoprecipitation of Hela labeling eIF4A3 using ab180573 at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180573).
Flow Cytometry - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)

Flow cytometric analysis of 2% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/160 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180573).
Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)

Immunofluorescent analysis of 4% paraformaldehyde Hela cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody at a dilution of 1/200.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180573).
Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)

Immunofluorescent analysis of 4% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180573).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling eIF4A3 with ab180573 at 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180573).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)

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