Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15656-290] to Androgen Receptor (AR-V7 specific)
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]
    See all Androgen Receptor (AR-V7 specific) primary antibodies

  • Description

    Rabbit monoclonal [EPR15656-290] to Androgen Receptor (AR-V7 specific)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 22Rv1 whole cell lysate. ICC/IF: 22Rv1 cells. Flow Cyt: 22Rv1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15656-290

  • Isotype

    IgG

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
    Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 22Rv1 cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in 22RV1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
    Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
    All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) at 1/1000 dilution

    Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate
    Lane 2 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
    Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 99 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:PMID: 30334814).

    Negative control: LNCaP and PC-3 (PMID:27117751, PMID: 30334814).

    Exposure time: 3 minutes.

  • Flow Cytometry - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
    Flow Cytometry - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 22Rv1 (human prostate carcinoma epithelial cell) cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
    Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) at 1/1000 dilution + 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysate was made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 81 seconds.

  • Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

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