Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
![Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)](https://www.abcam.com/ps/products/273/ab273500/Images/ab273500-1-anti-androgen-receptor-arv7-specific-antibody-epr15656290-immunocytochemistry-22rv1.jpg "Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15656-290] to Androgen Receptor (AR-V7 specific)
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]
See all Androgen Receptor (AR-V7 specific) primary antibodies -
Description
Rabbit monoclonal [EPR15656-290] to Androgen Receptor (AR-V7 specific) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 22Rv1 whole cell lysate. ICC/IF: 22Rv1 cells. Flow Cyt: 22Rv1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15656-290 -
Isotype
IgG
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 22Rv1 cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in 22RV1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)](https://www.abcam.com/ps/products/273/ab273500/Images/ab273500-393126-anti-androgen-receptor-arv7-specific-antibody-epr15656290-western-blot-22rv11.jpg)
Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) at 1/1000 dilution
Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate
Lane 2 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 99 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:PMID: 30334814).
Negative control: LNCaP and PC-3 (PMID:27117751, PMID: 30334814).
Exposure time: 3 minutes.
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![Flow Cytometry - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)](https://www.abcam.com/ps/products/273/ab273500/Images/ab273500-2-anti-androgen-receptor-arv7-specific-antibody-epr15656290-flow-cytometry-22rv1.jpg)
Flow Cytometry - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 22Rv1 (human prostate carcinoma epithelial cell) cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)](https://www.abcam.com/ps/products/273/ab273500/Images/ab273500-393125-anti-androgen-receptor-arv7-specific-antibody-epr15656290-western-blot-22rv1.jpg)
Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) at 1/1000 dilution + 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysate was made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 81 seconds.
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![Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)](https://www.abcam.com/ps/products/273/ab273500/Images/ab273500-5-benefits-of-recombinant-antibodies.png)
Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)
