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Cell Biology Other Antibodies

Anti-PNMAL1 antibody (ab224258)

Price and availability

341 740 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-PNMAL1 antibody (ab224258)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PNMAL1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PNMAL1 antibody
  • Description

    Rabbit polyclonal to PNMAL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human PNMAL1 aa 250-400.
    Database link: Q86V59

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: PNMAL1 over-expression - HEK-293T whole cell lysate; IHC: Human cerebral cortex tissue; ICC: RH-30 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-PNMAL1 antibody (ab224258)
    Western blot - Anti-PNMAL1 antibody (ab224258)
    All lanes : Anti-PNMAL1 antibody (ab224258) at 0.4 µg/ml

    Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : PNMAL1 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNMAL1 antibody (ab224258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNMAL1 antibody (ab224258)

    Immunohistochemical analysis of human cerebral cortex tissue labeling PNMAL1 in the cytoplasm and nucleus of neuronal cells with ab224258 at 1/200 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

     

     

    Paraffin-embedded human  tissue stained for using ab224258 at 1/200 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-PNMAL1 antibody (ab224258)
    Immunocytochemistry/ Immunofluorescence - Anti-PNMAL1 antibody (ab224258)

    Immunofluorescent analysis of RH-30 (human bone marrow metastasis cell line) whole cell labeling PNMAL1 (green) in nucleoplasm and nuclear bodies with ab224258 at 2 µg/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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