Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)
![Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.com/ps/products/180/ab180184/Images/ab180184-208759-ab180184c.jpg "Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10245(2)] to MLF2 - C-terminal
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-MLF2 antibody [EPR10245(2)] - C-terminal
See all MLF2 primary antibodies -
Description
Rabbit monoclonal [EPR10245(2)] to MLF2 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MLF2 aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q15773 -
Positive control
- Jurkat, fetal brain and HepG2 lysates; Human colon and Human prostate tissues; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10245(2) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)
Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling MLF2 with ab180184 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.com/ps/products/180/ab180184/Images/ab180184-208757-ab180184.jpg)
Western blot - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)All lanes : Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : Fetal brain lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
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![Flow Cytometry - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.com/ps/products/180/ab180184/Images/ab180184-208761-ab180184d.jpg)
Flow Cytometry - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)Flow Cytometrical analysis of permeabilized Jurkat cells labeling MLF2 with ab180184 at 1/10 (red) or a rabbit IgG negative (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.com/ps/products/180/ab180184/Images/ab180184-208758-ab180184b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling MLF2 with ab180184 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.com/ps/products/180/ab180184/Images/ab180184-5-benefits-of-recombinant-antibodies.png)
Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)
