Anti-p53 (acetyl K381) antibody (ab61241)
Key features and details
- Rabbit polyclonal to p53 (acetyl K381)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p53 (acetyl K381) antibody
See all p53 primary antibodies -
Description
Rabbit polyclonal to p53 (acetyl K381) -
Host species
Rabbit -
Specificity
ab61241 detects endogenous levels of p53 only when acetylated at lysine 381. Human: Lys381; Mouse: Lys375; Rat: Lys379. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Synthetic peptide corresponding to Human p53 (internal sequence) (acetyl K381). Synthetic acetylated peptide derived from human p53 around the acetylation site of lysine 381 (RHKAcKL).
Database link: P04637
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific acetylated peptide. The antibody against non acetylated peptide was removed by chromatography using non acetylated peptide corresponding to the acetylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K381) antibody (ab61241)
Immunofluorescence analysis of HeLa cells, using p53 (acetyl K381) antibody (ab61241) at 1/500 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide (blocking peptide).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (acetyl K381) antibody (ab61241)Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using p53 (acetyl K381) antibody (ab61241) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide (blocking peptide).
