Anti-DNA Ligase III/LIG3 antibody (ab224820)
Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies -
Description
Rabbit polyclonal to DNA Ligase III/LIG3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human DNA Ligase III/LIG3 aa 800-850. The exact sequence is proprietary. (CAA59230.1).
Database link: P49916-3 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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General notes
This product was previously labelled as DNA Ligase III
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab224820 was affinity purified using an epitope specific to DNA Ligase III/LIG3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DNA Ligase III/LIG3 antibody (ab224820)All lanes : Anti-DNA Ligase III/LIG3 antibody (ab224820) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-DNA Ligase III/LIG3 antibody (ab224820)DNA Ligase III/LIG3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer) with ab224820 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224820 at 1 µg/ml.
Lane 1: ab224820 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
