Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 02, 2021

Anti-LIG1 antibody [EPR12464] (ab177946)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12464] to LIG1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-LIG1 antibody [EPR12464]
See all LIG1 primary antibodies
Description

Rabbit monoclonal [EPR12464] to LIG1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human LIG1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P18858
Positive control
- WB: HeLa, Jurkat and Molt4 cell lysates IHC-P: Human breast and thyroid gland tissues ICC/IF: HeLa cells.
General notes
This product was previously labelled as DNA Ligase I

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12464
Isotype

IgG
Research areas

Western blot - Anti-LIG1 antibody [EPR12464] (ab177946)

All lanes : Anti-LIG1 antibody [EPR12464] (ab177946) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Molt 4 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (ab177946)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling LIG1 with ab177946 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (ab177946)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling LIG1 with ab177946 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody [EPR12464] (ab177946)

Immunofluorescent analysis of HeLa cells labeling LIG1 with ab177946 at 1/100 dilution.
Anti-LIG1 antibody [EPR12464] (ab177946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com