Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LIG1 antibody [EPR12464] (ab177946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12464] to LIG1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-LIG1 antibody [EPR12464]
    See all LIG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12464] to LIG1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LIG1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P18858

  • Positive control

    • WB: HeLa, Jurkat and Molt4 cell lysates IHC-P: Human breast and thyroid gland tissues ICC/IF: HeLa cells.

  • General notes

     

     

     This product was previously labelled as DNA Ligase I

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12464

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LIG1 antibody [EPR12464] (ab177946)
    Western blot - Anti-LIG1 antibody [EPR12464] (ab177946)
    All lanes : Anti-LIG1 antibody [EPR12464] (ab177946) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Molt 4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 102 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (ab177946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (ab177946)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling LIG1 with ab177946 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (ab177946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (ab177946)

    Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling LIG1 with ab177946 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody [EPR12464] (ab177946)
    Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody [EPR12464] (ab177946)

    Immunofluorescent analysis of HeLa cells labeling LIG1 with ab177946 at 1/100 dilution.

  • Anti-LIG1 antibody [EPR12464] (ab177946)
    Anti-LIG1 antibody [EPR12464] (ab177946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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