Anti-DNA Ligase III/LIG3 antibody (ab96576)
Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies -
Description
Rabbit polyclonal to DNA Ligase III/LIG3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DNA Ligase III/LIG3 aa 765-999 (internal sequence).
Database link: NP_039269 -
Positive control
- WB: A431, HeLa, HepG2, MOLT4, Raji cells IHC-P: H441 Xenograft
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General notes
This product was previously labelled as DNA Ligase III
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-DNA Ligase III/LIG3 antibody (ab96576) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 113 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of Ligase III in paraformaldehyde fixed A431, using ab96576 at a 1/200 dilution.
Lower image (2): merged with DNA probe. -
Immunohistochemical analysis of Ligase III in paraffin embedded H441 Xenograft, using ab96576 at a 1/100 dilution.