Anti-DNA Ligase III/LIG3 antibody (ab223739)
Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies -
Description
Rabbit polyclonal to DNA Ligase III/LIG3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DNA Ligase III/LIG3 aa 250-400.
Database link: P49916 -
Positive control
- IHC-P: Human testis, prostate, cerebral cortex, cerebellum tissue. WB: A549 whole cell lysates. ICC: U-251 MG whole cells.
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General notes
This product was previously labelled as DNA Ligase III
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DNA Ligase III/LIG3 antibody (ab223739)Anti-DNA Ligase III/LIG3 antibody (ab223739) at 0.4 µg/ml + A549 (Human lung carcinoma cell line) whole cell lysate
Predicted band size: 113 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (ab223739)Immunohistochemistry analysis of human testis tissue labeling LIG3 in the nucleus of cells in seminiferous ducts with ab223739 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-DNA Ligase III/LIG3 antibody (ab223739)Immunocytochemical analysis of U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cells labeling for DNA Ligase III/LIG3 (green) in the nucleoplasm with ab223739 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (ab223739)Immunohistochemistry analysis of human cerebral cortex tissue labeling LIG3 in the nucleus of neurons with ab223739 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (ab223739)Immunohistochemistry analysis of human prostate tissue labeling LIG3 in the nucleus of glandular cells with ab223739 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (ab223739)Immunohistochemistry analysis of human cerebellum tissue labeling LIG3 in the nucleus of Purkinje cells with ab223739 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (ab223739)Immunohistochemistry analysis of human skeletal muscle tissue labeling LIG3 in the nucleus of myocytes with ab223739 at a 1/50 dilution. Negative control showing weak nuclear positivity.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
