Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)
![Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)](https://www.abcam.com/ps/products/184/ab184177/Images/ab184177-333647-anti-cullin-5-cul-5-antibody-epr14725-western-blot.jpg "Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14725] to Cullin 5/CUL-5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Cullin 5/CUL-5 antibody [EPR14725]
See all Cullin 5/CUL-5 primary antibodies -
Description
Rabbit monoclonal [EPR14725] to Cullin 5/CUL-5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Hela, MOLT4 or T47-D cell lysates. Human mouse and Rat heart tissue lysates.
-
General notes
Previously labelled as Cullin 5.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14725 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)All lanes : Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177) at 1/2000 dilution (Purified)
Lane 1 : Human heart lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
-
![Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)](https://www.abcam.com/ps/products/184/ab184177/Images/ab184177-216523-anti-cullin-5-cul-5-antibody-epr14725-western-blot.jpg)
Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)All lanes : Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : T47-D cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
-
![Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)](https://www.abcam.com/ps/products/184/ab184177/Images/ab184177-3-benefits-of-recombinant-antibodies.png)
Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)
