Anti-FBXO4/FBX4 antibody (ab230302)
Key features and details
- Rabbit polyclonal to FBXO4/FBX4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FBXO4/FBX4 antibody
See all FBXO4/FBX4 primary antibodies -
Description
Rabbit polyclonal to FBXO4/FBX4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant fragment corresponding to Human FBXO4/FBX4 aa 100 to the C-terminus.
Database link: Q9UKT5 -
Positive control
- WB: HEK-293T, HepG2 and HL-60 whole cell lysate. IP: FBXO4/FBX4 IP in HepG2 whole cell lysate. IHC-P: Human brain and heart tissue.
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General notes
This product was previously labelled as FBXO4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FBXO4/FBX4 antibody (ab230302)All lanes : Anti-FBXO4/FBX4 antibody (ab230302) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HL-60 human promyelocytic leukemia cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 44, 35 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO4/FBX4 antibody (ab230302)Paraffin-embedded human brain tissue stained for FBXO4/FBX4 with ab230302 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO4/FBX4 antibody (ab230302)Paraffin-embedded human heart tissue stained for FBXO4/FBX4 with ab230302 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-FBXO4/FBX4 antibody (ab230302)FBXO4/FBX4 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab230302.
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG, was used as secondary antibody at 1/50,000 dilution.
Lane 1: Rabbit monoclonal IgG (1 μg) instead of ab230302 in HepG2 whole cell lysate.
Lane 2: ab230302 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 20 μg (Input).
