Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E160] to CBL (phospho Y774) - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free
    See all CBL primary antibodies

  • Description

    Rabbit monoclonal [E160] to CBL (phospho Y774) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody detects Cbl phosphorylated on Tyrosine 774. 

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247260 is the carrier-free version of ab32446 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247260 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
    Western blot - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
    All lanes : Anti-CBL (phospho Y774) antibody [E160] (ab32446) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate + Pervanadate

    Predicted band size: 99 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32446, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
    This data was developed using ab32446, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of human cervical carcinoma using ab32446 at 1/25 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
    Flow Cytometry - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
    This data was developed using ab32446, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or Pervanadate-treated (red), using ab32446 at 1/20 dilution.
  • Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
    Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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