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FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)

Key features and details

  • FITC Mouse monoclonal [MEM-238] to M6PR (cation independent)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Overview

  • Product name

    FITC Anti-M6PR (cation independent) antibody [MEM-238]
    See all M6PR (cation independent) primary antibodies

  • Description

    FITC Mouse monoclonal [MEM-238] to M6PR (cation independent)

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant Vaccinia virus encoding Mannose 6 Phosphate Receptor (Cation independent) (CD222).

  • Epitope

    ab58841 recognizes an epitope between domains 2 and 5 of Mannose 6 Phosphate Receptor (Cation independent) (CD222).

  • Positive control

    • Jurkat whole cell lysate (ab7899)

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Properties

Images

  • Western blot - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
    Western blot - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
    Lane 1 : FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
    Lane 2 : Mouse IgG1 isotype control

    All lanes : Jurkat whole cell lysate

    Predicted band size: 275 kDa

  • Flow Cytometry - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
    Flow Cytometry - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
    Overlay histogram showing HeLa cells stained with ab58841 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58841, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

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