Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)
![Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)](https://www.abcam.com/ps/products/22/ab22674/Images/ab22674-32467-anti-proteasome-20s-alpha-123567-antibody-mcp231-immunofluorescence.jpg "Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)")
Key features and details
- Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] -
Description
Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Full length protein corresponding to Proteasome 20S alpha 1+2+3+5+6+7.
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Epitope
Reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to alpha type subunits, although not necessarily identical in all. In general this motif is phylogenetically preserved. -
Positive control
- Human placental proteasome.
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General notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Dilute antibody to working dilution in PBS , pH 7.2 - 7.4. Store at 4°C and use within 1 month. Do not freeze.
This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Primary antibody notes
This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7. -
Clonality
Monoclonal -
Clone number
MCP231 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)
ICC/IF image of ab22674 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22674, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)](https://www.abcam.com/ps/products/22/ab22674/Images/ab22674-32466-anti-proteasome-20s-alpha-123567-antibody-mcp231-flow-cytometry.jpg)
Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)Overlay histogram showing HepG2 cells stained with ab22674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22674, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
