Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)

Key features and details

  • Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231]

  • Description

    Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Full length protein corresponding to Proteasome 20S alpha 1+2+3+5+6+7.

  • Epitope

    Reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to alpha type subunits, although not necessarily identical in all. In general this motif is phylogenetically preserved.

  • Positive control

    • Human placental proteasome.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Dilute antibody to working dilution in PBS , pH 7.2 - 7.4. Store at 4°C and use within 1 month. Do not freeze.

    This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Primary antibody notes

    This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7.

  • Clonality

    Monoclonal

  • Clone number

    MCP231

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)

    ICC/IF image of ab22674 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22674, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)
    Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)

    Overlay histogram showing HepG2 cells stained with ab22674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22674, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.