Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
![Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)](https://www.abcam.com/ps/products/41/ab41616/Images/ab41616-54958-anti-dengue-virus-ns1-glycoprotein-antibody-dn3-western-blot.jpg "Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)")
Key features and details
- Mouse monoclonal [DN3] to Dengue Virus NS1 glycoprotein
- Suitable for: WB, ICC/IF
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
- Isotype: IgG1
Overview
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Product name
Anti-Dengue Virus NS1 glycoprotein antibody [DN3]
See all Dengue Virus NS1 glycoprotein primary antibodies -
Description
Mouse monoclonal [DN3] to Dengue Virus NS1 glycoprotein -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4 -
Immunogen
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.
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Positive control
- It is important to ensure that DEN4 infection is effective. DEN4 is notoriously difficult to grow to good titres and NS1 antigens will not be found if infection is not well established.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
DN3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
Lane 1 : Dengue Virus 1 infected C6/36 cell lysate (unheated)
Lane 2 : Dengue Virus 1 infected C6/36 cell lysate (boiled)
Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (unheated)
Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (boiled)
Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate (unheated)
Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate (boiled)
Lane 7 : Dengue Virus 3 infected C6/36 cell lysate (unheated)
Lane 8 : Dengue Virus 3 infected C6/36 cell lysate (boiled)
Lane 9 : Dengue Virus 4 infected C6/36 cell lysate (unheated)
Lane 10 : Dengue Virus 4 infected C6/36 cell lysate (boiled)
Predicted band size: 40, 80 kDa
Observed band size: 40,80 kDa why is the actual band size different from the predicted?
Recognises NS1 dimer (~80 kDa) in unheated samples and NS1 monomer (~40 kDa) when samples are boiled. -
![Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)](https://www.abcam.com/ps/products/41/ab41616/Images/ab41616-54957-anti-dengue-virus-ns1-glycoprotein-antibody-dn3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein NS1 (green). Cell nuclei are blue. -
![Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)](https://www.abcam.com/ps/products/41/ab41616/Images/ab41616-54956-anti-dengue-virus-ns1-glycoprotein-antibody-dn3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616) Image from Heaton NS et al, Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17345-50. Epub 2010 Sep 20, Fig 2. DOI 10.1073/pnas.1010811107ab41616 staining NS1 glycoprotein in Dengue Virus 2 infected Huh-7.5 cells by Immunocytochemistry/ Immunofluorescence.
Huh-7.5 cells were plated on glass coverslips, infected with Dengue Virus 2, fixed in methanol, and blocked, and antibody was added in 1× PBS + 0.1% saponin. Antibodies used include FASN polyclonal, left image (ab3844) or NS1 monoclonal antibody, middle image (ab41616). Secondary antibodies were species-appropriate Alexa-Fluor 488 or 594 secondary antibody. Stained coverslips were mounted with DAPI, right merged image.
