Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)
![Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)](https://www.abcam.com/ps/products/41/ab41623/Images/ab41623-208656-anti-dengue-virus-ns1-glycoprotein-antibody-dn2-immunofluorescence.jpg "Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)")
Key features and details
- Mouse monoclonal [DN2] to Dengue Virus NS1 glycoprotein
- Suitable for: WB, ICC/IF
- Reacts with: Dengue virus 2
- Isotype: IgG1
Overview
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Product name
Anti-Dengue Virus NS1 glycoprotein antibody [DN2]
See all Dengue Virus NS1 glycoprotein primary antibodies -
Description
Mouse monoclonal [DN2] to Dengue Virus NS1 glycoprotein -
Host species
Mouse -
Specificity
Recognises NS1 from both Dengue Virus 2 strains, 16681 and NGC. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Dengue virus 2 -
Immunogen
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
DN2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623) This image is courtesy of an Abreview submitted by Lee Gehrke
ab41623 staining Dengue Virus NS1 glycoprotein (green) in Human BHK cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed and permeabilized with CytoFix/CytoPerm and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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![Western blot - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)](https://www.abcam.com/ps/products/41/ab41623/Images/ab41623-54973-anti-dengue-virus-ns1-glycoprotein-antibody-dn2-western-blot.jpg)
Western blot - Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)
Lane 1 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (unheated)
Lane 2 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (boiled)
Lane 3 : Dengue Virus 2 (16681)infected C6/36 cell lysate (unheated)
Lane 4 : Dengue Virus 2 (16681)infected C6/36 cell lysate (boiled)
Predicted band size: 40 kDa
Observed band size: 40,80 kDa why is the actual band size different from the predicted?
NS1 exists as a dimer (~80 kDa) in unheated samples but is dissociated into a monomer (~40 kDa) when samples are boiled. Ab41623 recognised both forms of NS1.
