Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23539-255] to GABA A Receptor alpha 1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GABA A Receptor alpha 1 antibody [EPR23539-255]
See all GABA A Receptor alpha 1 primary antibodies -
Description
Rabbit monoclonal [EPR23539-255] to GABA A Receptor alpha 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatIP MouseWB MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse and rat cerebrum tissue. WB: Mouse and rat brain, cerebellum and hippocampus tissue lysate. Human brain and cerebellum tissue lysate. IP: Mouse cerebellum tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23539-255 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GABA A Receptor alpha 1 with ab252430 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrumis observed (PMID:29467616). The section was incubated with ab252430 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human cerebellum tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).
Exposure time: 3 minutes.
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GABA A Receptor alpha 1 was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10µg with ab252430 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252430 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10µg.
Lane 2: ab252430 IP in mouse cerebellum tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252430 in mouse cerebellum tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 33 seconds.
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Mouse hippocampus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot of Lane 1-2 was developed using a higher sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).
Exposure time: Lane 1-2: 156 seconds; Lane 3: 3 minutes.
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat cerebellum tissue lysate
Lane 3 : Rat hippocampus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).
Exposure time: 3 minutes.
-
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GABA A Receptor alpha 1 with ab252430 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum is observed (PMID:29467616). The section was incubated with ab252430 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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