Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23539-255] to GABA A Receptor alpha 1
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GABA A Receptor alpha 1 antibody [EPR23539-255]
    See all GABA A Receptor alpha 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23539-255] to GABA A Receptor alpha 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat cerebrum tissue. WB: Mouse and rat brain, cerebellum and hippocampus tissue lysate. Human brain and cerebellum tissue lysate. IP: Mouse cerebellum tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GABA A Receptor alpha 1 with ab252430 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrumis observed (PMID:29467616). The section was incubated with ab252430 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human cerebellum tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)

    GABA A Receptor alpha 1 was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10µg with ab252430 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252430 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse cerebellum tissue lysate 10µg.

    Lane 2: ab252430 IP in mouse cerebellum tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252430 in mouse cerebellum tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 33 seconds.

     

  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse cerebellum tissue lysate
    Lane 3 : Mouse hippocampus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 51 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot of Lane 1-2 was developed using a higher sensitivity ECL substrate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).

    Exposure time: Lane 1-2: 156 seconds; Lane 3: 3 minutes.

  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat cerebellum tissue lysate
    Lane 3 : Rat hippocampus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 51 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GABA A Receptor alpha 1 with ab252430 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum is observed (PMID:29467616). The section was incubated with ab252430 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
    Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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