Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13042(B)] to SDHB - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free
    See all SDHB primary antibodies

  • Description

    Rabbit monoclonal [EPR13042(B)] to SDHB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250046 is the carrier-free version of ab178423. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250046 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    Western blot - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/5000 dilution

    Lane 1 : Wild-type HEK293 whole cell lysate
    Lane 2 : SDHB knockout HEK293 whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 32 kDa



    This data was developed using ab178423, the same antibody clone in a different buffer formulation.

    Lanes 1 - 3: Merged signal (red and green). Green - ab178423 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab178423 was shown to recognize SDHB in wild-type HEK 293 cells as signal was lost at the expected MW in SDHB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab178423 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    Western blot - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 32 kDa



    This data was developed using ab178423, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    This data was developed using ab178423, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SDHB with ab178423 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    This data was developed using ab178423, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SDHB with ab178423 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    Flow Cytometry - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    This data was developed using ab178423, the same antibody clone in a different buffer formulation.Flow Cytometric analysis of permeabilized HepG2 cells labeling SDHB with ab178423 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
  • Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    This data was developed using ab178423, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) A431 cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178423 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
  • Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)
    Anti-SDHB antibody [EPR13042(B)] - BSA and Azide free (ab250046)

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