Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Platelets, Serum, Tissue Extracts, Urine
- Sensitivity: 0.5 mU/ml
Overview
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Product name
Glutathione Peroxidase Assay Kit (Colorimetric)
See all Glutathione Peroxidase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Platelets, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
0.5 mU/ml -
Assay time
0h 40m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Glutathione Peroxidase Assay Kit (Colorimetric) ab102530 can be used to quantitate the activity of all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.
In the glutathione peroxidase assay protocol, glutathione peroxidase (GPx) oxidizes GSH to produce GSSG as part of the reaction in which it reduces cumene hydroperoxide. Glutathione reductase (GR) then reduces the GSSG to produce GSH, and in the same reaction consumes NADPH. The decrease of NADPH (measured at OD=340 nm) is proportional to GPx activity.
The assay has a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.
Glutathione peroxidase assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 15 min at room temp to deplete all GSSG in sample
- add cumene hydroperoxide
- analyze with microplate reader immediately, and after at least 5 min -
Notes
The Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes plays and important role in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Cumene Hydroperoxide Yellow 1 vial Glutathione Brown 1 vial Glutathione Reductase Green 1 vial GPx Assay Buffer WM 1 x 50ml GPx Positive Control Red 1 vial NADPH Blue 1 vial -
Research areas
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Relevance
Glutathione Peroxidase (GPx, EC 1.11.1.9) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. -
Alternative names
- GPX
Images
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Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530) George, Akash K et al., Scientific reports?vol. 8,1 5158., Fig 4, doi:10.1038/s41598-018-23568-z
Scatter dot plots representing the levels of glutathione peroxidase (GPx) in brain tissue in different mice groups.
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Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530) Liu, Baohai et al., Frontiers in pharmacology?vol. 9 347., Fig 8, doi:10.3389/fphar.2018.00347The effects of PCSE on the activity of GPX in a mouse UC model. -
Functional Studies - Glutathione Peroxidase Assay Kit (ab102530)Standard curve: mean of duplicates (+/- SD) with background reads subtracted -
Functional Studies - Glutathione Peroxidase Assay Kit (ab102530)Glutathione Peroxidase Activity measured in biological fluids showing quantity (nmol) per 1 mL after 10 min of incubation. Samples diluted 1-27 fold.
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Functional Studies - Glutathione Peroxidase Assay Kit (ab102530)Glutathione Peroxidase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 10 min of incubation. Undiluted sample contained 2.5e6 cells per well. Samples were diluted 1-9 fold.
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Functional Studies - Glutathione Peroxidase Assay Kit (ab102530)Tests example obtained using ab102530
