Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab48019)
Key features and details
- Goat polyclonal to Cytochrome P450 17A1/CYP17A1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome P450 17A1/CYP17A1 antibody
See all Cytochrome P450 17A1/CYP17A1 primary antibodies -
Description
Goat polyclonal to Cytochrome P450 17A1/CYP17A1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Hamster, Cat, Dog, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human Cytochrome P450 17A1/CYP17A1 aa 134-144 (internal sequence).
Sequence:LFKDGDQKLEK
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Positive control
- Human ovary lysates.
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General notes
This product was previously labelled as Cytochrome P450 17A1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab48019)Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab48019) at 0.3 µg/ml + 35µg Human ovary lysates in RIPA buffer
Secondary
anti-goat-HRP at a 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab48019)ab48019 (3.8µg/ml) staining of paraffin embedded Human Adrenal Gland shows strong granular staining of the cytoplasm in medulla cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
