Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17207] to PRPF4 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRPF4 antibody [EPR17207] - BSA and Azide free
    See all PRPF4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17207] to PRPF4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251263 is the carrier-free version of ab198998. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251263 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Western blot - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/20000 dilution

    Lane 1 : Raji (Human Burkitt's lymphoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa


    Exposure time: 3 minutes


    This data was developed using ab198998, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)

    This data was developed using ab198998, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Human transitional cell carcinoma of bladder tissue was observed (Subcellular location - Nucleus speckle [UniProt]). Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Western blot - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa


    Exposure time: 3 minutes


    This data was developed using ab198998, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)

    This data was developed using ab198998, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Human kidney tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Western blot - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophages transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa


    Exposure time: 15 seconds


    This data was developed using ab198998, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    This data was developed using ab198998, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Rat cerebral cortex tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    This data was developed using ab198998, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Mouse cardiac muscle tissuewas observed (Subcellular location - Nucleus speckle [UniProt]).
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Flow Cytometry - Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    This data was developed using ab198998, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labelling PRPF4 (red) with purified ab198998 at dilution of 1/70. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)
    Anti-PRPF4 antibody [EPR17207] - BSA and Azide free (ab251263)

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