Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)
![Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)](https://www.abcam.com/ps/products/251/ab251286/Images/ab251286-405846-anti-map17-antibody-epr16827-bsa-and-azide-free-western-blot.jpg "Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16827] to Map17 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Map17 antibody [EPR16827] - BSA and Azide free
See all Map17 primary antibodies -
Description
Rabbit monoclonal [EPR16827] to Map17 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251286 is the carrier-free version of ab199540. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251286 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16827 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)All lanes : Anti-Map17 antibody [EPR16827] (ab199540) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 12 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis data was developed using ab199540, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID: 10951271.
Human fetal brain, Human fetal heart, Human fetal spleen represent negative controls for Map17.
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![Immunoprecipitation - Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)](https://www.abcam.com/ps/products/251/ab251286/Images/ab251286-2-anti-map17-antibody-epr16827-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)This data was developed using ab199540, the same antibody clone in a different buffer formulation.Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab199540 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal kidney whole cell lysate 10ug (Input). Lane 2: ab199540 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds. -
![Immunoprecipitation - Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)](https://www.abcam.com/ps/products/251/ab251286/Images/ab251286-399681-anti-map17-antibody-epr16827-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)This data was developed using ab199540, the same antibody clone in a different buffer formulation.
Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/300 dilution. Western blot was performed from the immunoprecipitate using anti-Map17 (ab156014) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell lysate 10ug (Input). Lane 2: ab199540 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)](https://www.abcam.com/ps/products/251/ab251286/Images/ab251286-4-benefits-of-recombinant-antibodies.png)
Anti-Map17 antibody [EPR16827] - BSA and Azide free (ab251286)