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Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
    See all Cytochrome P450 17A1/CYP17A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cytochrome P450 17A1/CYP17A1 aa 100-200. The exact sequence is proprietary.
    Database link: P05093

  • Positive control

    • WB: Human adrenal gland, mouse and rat heart lysates, human fetal heart lysate, SK-OV-3, HeLa and Jurkat lysates; Flow Cyt: HeLa cells ICC/IF: HeLa cells

  • General notes

     This product was previously labelled as Cytochrome P450 17A1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 17A1/CYP17A1 with Purified ab125022 at 1:100 dilution (3.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
    Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
    Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/2000 dilution (purified) + Human fetal heart lysates at 15 µg

    Predicted band size: 57 kDa

  • Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
    Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    ab125022 (purified) at 1:30 dilution (2µg) immunoprecipitating Cytochrome P450 17A1/CYP17A1 in Human fetal heart lysate.
    Lane 1 (input): Human fetal heart lysate 10µg
    Lane 2 (+): ab125022 & Human fetal heart lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125022 in Human fetal heart lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
    Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
    All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution

    Lane 1 : Human adrenal gland lysate
    Lane 2 : SK-OV-3 lysate
    Lane 3 : HeLa lysate
    Lane 4 : Jurkat lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
    Flow Cytometry - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1/CYP17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
    Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

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