Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

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Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
Suitable for: WB, IP, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
See all Cytochrome P450 17A1/CYP17A1 primary antibodies
Description

Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cytochrome P450 17A1/CYP17A1 aa 100-200. The exact sequence is proprietary.
Database link: P05093
Positive control
- WB: Human adrenal gland, mouse and rat heart lysates, human fetal heart lysate, SK-OV-3, HeLa and Jurkat lysates; Flow Cyt: HeLa cells ICC/IF: HeLa cells
General notes
This product was previously labelled as Cytochrome P450 17A1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6293
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 17A1/CYP17A1 with Purified ab125022 at 1:100 dilution (3.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/2000 dilution (purified) + Human fetal heart lysates at 15 µg

Predicted band size: 57 kDa
Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

ab125022 (purified) at 1:30 dilution (2µg) immunoprecipitating Cytochrome P450 17A1/CYP17A1 in Human fetal heart lysate.
Lane 1 (input): Human fetal heart lysate 10µg
Lane 2 (+): ab125022 & Human fetal heart lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125022 in Human fetal heart lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution

Lane 1 : Human adrenal gland lysate
Lane 2 : SK-OV-3 lysate
Lane 3 : HeLa lysate
Lane 4 : Jurkat lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1/CYP17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

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