Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6294(2)] to Cytochrome P450 17A1/CYP17A1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)]
See all Cytochrome P450 17A1/CYP17A1 primary antibodies
Description

Rabbit monoclonal [EPR6294(2)] to Cytochrome P450 17A1/CYP17A1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human adrenal gland lysate, mouse and rat testis lysate. IHC-P: Human and mouse testis tissues.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as Cytochrome P450 17A1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6294(2)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Cytochrome P450 17A1/CYP17A1 with ab134910 at 1/250 followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Cytoplasmic staining on mouse Leydig cells.The section was incubated with ab134910 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910) at 1/1000 dilution

Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 57 kDa
Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910) at 1/10000 dilution + Human adrenal gland lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Cytochrome P450 17A1/CYP17A1 with ab134910 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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