Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7529] to CYP27A1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free
    See all CYP27A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7529] to CYP27A1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Caco 2, Human fetal liver and Mouse liver lysates; Human kidney and liver tissues; HepG2 cells.

  • General notes

    ab214800 is the carrier-free version of ab126785.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)

    ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
    Flow Cytometry - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)

    Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)

    ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
    Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
    ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

  • OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
    OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

  • Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
    Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)

© 2021 Astana Biomed Group LLP. All rights reserved.