Anti-CYP21A2 antibody (ab230327)
Key features and details
- Rabbit polyclonal to CYP21A2
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP21A2 antibody
See all CYP21A2 primary antibodies -
Description
Rabbit polyclonal to CYP21A2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CYP21A2 aa 216-246 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P08686 -
Positive control
- IHC-P: Human adrenal tissue. ICC/IF: HEK-293 cells. WB: MDA-MB435 cell lysate. Flow Cyt: MDA-MB231 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (ab230327)
Formalin-fixed, paraffin-embedded human adrenal tissue stained for CYP21A2 using ab230327 at 1/50 dilution in imunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CYP21A2 antibody (ab230327)HEK-293 (human epithelial cell line from embryonic kidney) cells stained for CYP21A2 using ab230327 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) in confocal immunofluorescence.
Nucelar counterstain: DAPI (blue).
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Western blot - Anti-CYP21A2 antibody (ab230327)Anti-CYP21A2 antibody (ab230327) at 1/1000 dilution + MDA-MB-435S (Human ductal carcinoma cell line) cell lysate at 35 µg
Predicted band size: 56 kDa
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Flow Cytometry - Anti-CYP21A2 antibody (ab230327)Flow cytometric analysis of MDA-MB231 (human breast adenocarcinoma cell line) cells labeling CYP21A2 with ab230327 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
