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Anti-CYP27A1 antibody [EPR7529] (ab126785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7529] to CYP27A1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CYP27A1 antibody [EPR7529]
    See all CYP27A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7529] to CYP27A1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in Human CYP27A1 (UniProt Q02318).

  • Positive control

    • WB: HepG2, Caco 2, human fetal liver and mouse liver lysates. IHC-P: Human kidney and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    All lanes : Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : Caco 2 lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Mouse liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)

    ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.
  • Flow Cytometry - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    Flow Cytometry - Anti-CYP27A1 antibody [EPR7529] (ab126785)

    Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)

    ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (ab126785)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CYP27A1 antibody [EPR7529] (ab126785)
    Anti-CYP27A1 antibody [EPR7529] (ab126785)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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