Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 120 614 ₸

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Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8298(B)] to CUG-BP1
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-CUG-BP1 antibody [EPR8298(B)]
See all CUG-BP1 primary antibodies
Description

Rabbit monoclonal [EPR8298(B)] to CUG-BP1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human CUG-BP1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q92879
Positive control
- WB: HEK-293T, HeLa and SH-SY5Y cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 3.76 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8298(B)
Isotype

IgG
Research areas

Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)

All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CELF1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Lanes 1-4: Merged signal (red and green). Green - ab129115 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.

ab129115 Anti-CUG-BP1 antibody [EPR8298(B)] was shown to specifically react with CUG-BP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266086 (knockout cell lysate ab257390) was used. Wild-type and CUG-BP1 knockout samples were subjected to SDS-PAGE. ab129115 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)

All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa
OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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