Anti-CUG-BP1 antibody [3B1] (ab9549)
![Anti-CUG-BP1 antibody [3B1] (ab9549)](https://www.abcam.com/ps/products/9/ab9549/Images/ab9549-263637-anti-cug-bp1-antibody-3b1-immunofluorescence.jpg "Anti-CUG-BP1 antibody [3B1] (ab9549)")
Key features and details
- Mouse monoclonal [3B1] to CUG-BP1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CUG-BP1 antibody [3B1]
See all CUG-BP1 primary antibodies -
Description
Mouse monoclonal [3B1] to CUG-BP1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
corresponding to CUG-BP1.
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Positive control
- WB: HeLa, HEK-293T and SHSY-5Y cell lysates; Human kidney and brain tissue lysates. IF/ICC: MCF7 cells. IHC: Human Breast cancer tissue
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3B1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CUG-BP1 antibody [3B1] (ab9549)
ab9549 stained MCF7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9549 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-CUG-BP1 antibody [3B1] (ab9549)](https://www.abcam.com/ps/products/9/ab9549/Images/ab9549-263271-anti-cug-bp1-antibody-3b1-western-blot.jpg)
Western blot - Anti-CUG-BP1 antibody [3B1] (ab9549)All lanes : Anti-CUG-BP1 antibody [3B1] (ab9549) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 20 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUG-BP1 antibody [3B1] (ab9549)](https://www.abcam.com/ps/products/9/ab9549/Images/ab9549-263635-anti-cug-bp1-antibody-3b1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUG-BP1 antibody [3B1] (ab9549)IHC image of CUG-BP1 staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9549, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
