Anti-Y14 antibody (ab229573)
Key features and details
- Rabbit polyclonal to Y14
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Y14 antibody
See all Y14 primary antibodies -
Description
Rabbit polyclonal to Y14 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish
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Immunogen
Recombinant fragment within Human Y14 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y5S9 -
Positive control
- IHC-P: Rat kidney and adrenal gland tissues. ICC/IF: HeLa cells. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Y14 antibody (ab229573)All lanes : Anti-Y14 antibody (ab229573) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 19 kDa15% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Y14 antibody (ab229573)Paraffin-embedded rat kidney tissue stained for Y14 using ab229573 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Y14 antibody (ab229573)Paraffin-embedded rat adrenal gland tissue stained for Y14 using ab229573 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Y14 antibody (ab229573)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Y14 (green) using ab229573 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
