Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [2A] to Connexin 32 / GJB1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free
See all Connexin 32 / GJB1 primary antibodies -
Description
Mouse monoclonal [2A] to Connexin 32 / GJB1 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human, mouse and rat liver tissue lysate.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab256132 is the carrier-free version of ab78052. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256132 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2A -
Isotype
IgG1 -
Research areas
Images
-
All lanes : Anti-Connexin 32 / GJB1 antibody [2A] (ab78052) at 1.318 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 46,100 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThe molecular observed was in consist with published literatures (PMID 11226030, PMID: 11245683, PMID: 12403817).
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78052).
-
Anti-Connexin 32 / GJB1 antibody [2A] (ab78052) at 1.318 µg/ml + Rat liver tissue lysate at 20 µg
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 46,100 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThe molecular observed was in consist with published literatures (PMID 11226030, PMID: 11245683, PMID: 12403817).
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78052).
-