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Neuroscience Neurotransmission Receptors / Channels More Ion Channels

Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)

Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [2A] to Connexin 32 / GJB1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free
    See all Connexin 32 / GJB1 primary antibodies
  • Description

    Mouse monoclonal [2A] to Connexin 32 / GJB1 - BSA and Azide free
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat liver tissue lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab256132 is the carrier-free version of ab78052. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256132 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    2A
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • More Ion Channels

Images

  • Western blot - Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)
    Western blot - Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)
    All lanes : Anti-Connexin 32 / GJB1 antibody [2A] (ab78052) at 1.318 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 32 kDa
    Observed band size: 46,100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    The molecular observed was in consist with published literatures (PMID 11226030, PMID: 11245683, PMID: 12403817).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78052).

  • Western blot - Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)
    Western blot - Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)
    Anti-Connexin 32 / GJB1 antibody [2A] (ab78052) at 1.318 µg/ml + Rat liver tissue lysate at 20 µg

    Secondary
    Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 32 kDa
    Observed band size: 46,100 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    The molecular observed was in consist with published literatures (PMID 11226030, PMID: 11245683, PMID: 12403817).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78052).

  • Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)
    Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free (ab256132)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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