Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)
![Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)](https://www.abcam.com/ps/products/249/ab249397/Images/ab249397-410386-anti-inppl1-ship-2-antibody-epr10955-bsa-and-azide-free-western-blot.jpg "Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10955] to INPPL1/SHIP-2 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free
See all INPPL1/SHIP-2 primary antibodies -
Description
Rabbit monoclonal [EPR10955] to INPPL1/SHIP-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249397 is the carrier-free version of ab166916. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249397 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10955 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)
This data was developed using ab166916, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: INPPL1/SHIP-2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab166916 observed at 139 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab166916 was shown to specifically react with INPPL1/SHIP-2 in wild-type HAP1 cells. No band was observed when INPPL1/SHIP-2 knockout samples were examined. Wild-type and INPPL1/SHIP-2 knockout samples were subjected to SDS-PAGE. ab166916 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)](https://www.abcam.com/ps/products/249/ab249397/Images/ab249397-4-anti-inppl1-ship-2-antibody-epr10955-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)This data was developed using ab166916, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling INPPL1/SHIP-2 with ab166916 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)](https://www.abcam.com/ps/products/249/ab249397/Images/ab249397-405841-anti-inppl1-ship-2-antibody-epr10955-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)](https://www.abcam.com/ps/products/249/ab249397/Images/ab249397-2-anti-inppl1-ship-2-antibody-epr10955-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)This data was developed using ab166916, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling INPPL1/SHIP-2 with ab166916 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)](https://www.abcam.com/ps/products/249/ab249397/Images/ab249397-1-anti-inppl1-ship-2-antibody-epr10955-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397) -
![Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)](https://www.abcam.com/ps/products/249/ab249397/Images/ab249397-6-benefits-of-recombinant-antibodies.png)
Anti-INPPL1/SHIP-2 antibody [EPR10955] - BSA and Azide free (ab249397)
